Bench Scientist job in Cambridge

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Massachusetts (MA), Cambridge

Job Code:	24-01231
Employer:	Aequor
Category:	Research and Development
Location
Country:	United States
State:	Massachusetts (MA)
City:	Cambridge
Post Date:	04/17/2024
Description
Job Description : BioMedicine Design (BMD) is seeking a highly motivated Biochemist/Molecular Biologist to join Biotherapeutic Expression and Purification group (BEP) in Cambridge, MA. Qualified candidate will play an essential role in a high-energy DNA production and protein expression research laboratory. The group supports non-GMP transient protein lead production across the companies diverse biotherapeutic discovery portfolio to enable the research units and their pre-clinical therapeutic in-vitro and in-vivo needs. The BEP DNA production and protein expression group is looking for a laboratory technician to provide DNA production support for the protein expression and purification activities in BEP. The candidate is expected to have a strong background in molecular biology techniques and experience with nucleic acid production and analysis. The successful candidate will grow bacterial cultures and perform large-scale DNA production activities following standardized protocols. The candidate will be a key contributor to the scientific team responsible for protein expression and purification. Minimum education experience required: B.Sc in biological/life sciences, College grad acceptable or Associates / Certificate in Biotechnology, 1-2 years exp or High School diploma with at least 2 years of relevant laboratory research school Role Responsibilities Production of plasmid DNA to support transient recombinant protein expression. Conducting routine molecular biology techniques including cell transformations, day cultures, bacterial cultures, Gibson assembly, and digestions. Maintaining reagent and DNA inventory. Operate and maintain automated liquid handlers for plasmid purification, as well as downstream QC applications like NGS and DNA normalizations. Conducting routine DNA characterization and quality control testing using techniques such as gel electrophoresis, fragment analysis, nanodrop, endotoxin testing, and preparation of samples for sequencing analysis (Sanger and NGS). Maintaining daily work records in an organized electronic lab notebook. The candidate should also be proficient in using an online database for inventory management. Effectively communicating with team members to meet strict schedules and deadlines Required Skills: Production of plasmid DNA Conducting routine molecular biology techniques Maintaining reagent and DNA inventory. Preferred Skills: Handling Automations Familiarity NGS "Nanopore " Nice to Have Familiarity with Golden Gate or Gibson assembly Interview Process: Multiple Panel interviews with camera on. Position Comments visible to Supplier: Previous experience in a CRO role, preferred.